Aspartame, a non-carbohydrate compound, is a kind of artificial sweetener and it is the most common sugar substitute in beverage, vitamin tablet and chewing.
Table 1 Information of Analyte
HPLC purity of methanol; Watsons water; carrot juice was provided by customers.
Weigh 2 g carrot juice and dissolve it to a certain volume of 25 mL using water. Centrifuge at 4000 r/min for 5 min, and filter the supernate through 0.45 mm filter followed by HPLC analysis.
HPLC Column: Venusil C18 Plus; 5 μm, 120 Å, 4.6 × 250 mm;
Mobile phase: Water : Methanol = 61:39;
Detection: 208 nm;
Flow rate: 0.8 mL/min;
Column temperature: 25 °C;
Injection: 20 mL.
Results and Discussion
Venusil C18 Plus HPLC column was adopted to detect the Aspartame in carrot juice. Table 2 and the following Figures show that the result can support analysis requirements.
Table 2 Performance of VenusilÒ C18 Plus in determination of Aspartame in carrot juice
Number of theoretical number
Standard sample of Aspartame
Carrot juice sample
Figure 1 Chromatogram of Aspartame standard sample
Figure 2 Chromatogram of carrot juice sample
An effective HPLC method was developed for determination of Aspartame in carrot juice using VenusilÒ C18 Plus. Excellent separation was gained without fear of matrix influence. Typical method performance results were well within acceptable criteria.
Venusil® C18 Plus
5 μm, 120 Å, 4.6×250 mm
1.5 mL vials
Screw neck vials, 12 × 32 mm
Caps and Septa
Screw neck cap, center hole; red silicone/ white PTEE septa, slitted
Monofilm, 13 mm, 0.45 μm
Disposable Needle-Free injection systems
2 mL, 100/pk