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Determination of Aspartame in carrot juice using Venusil C18 Plus

Application Introduction

Aspartame, a non-carbohydrate compound, is a kind of artificial sweetener and it is the most common sugar substitute in beverage, vitamin tablet and chewing.

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Introduction

Aspartame, a non-carbohydrate compound, is a kind of artificial sweetener and it is the most common sugar substitute in beverage, vitamin tablet and chewing.

Table 1 Information of Analyte

Name

Structure

Formula

Molecular Weight

CAS

   Aspartame

 C14H18N2O5  

294.3031

22839-47-0

Experimental

Material

HPLC purity of methanol; Watsons water; carrot juice was provided by customers.

Weigh 2 g carrot juice and dissolve it to a certain volume of 25 mL using water. Centrifuge at 4000 r/min for 5 min, and filter the supernate through 0.45 mm filter followed by HPLC analysis.

Instrumentation

HPLC Column: Venusil C18 Plus; 5 μm, 120 Å, 4.6 × 250 mm;

Mobile phase: Water : Methanol = 61:39;

Detection: 208 nm;

Flow rate: 0.8 mL/min;

Column temperature: 25 °C;

Injection: 20 mL.

Results and Discussion

Venusil C18 Plus HPLC column was adopted to detect the Aspartame in carrot juice. Table 2 and the following Figures show that the result can support analysis requirements.

Table 2 Performance of VenusilÒ C18 Plus in determination of Aspartame in carrot juice

Sample

Retention Time

Number of theoretical number

Tailing factor

Comments

Standard sample of Aspartame

13.939

14369

1.034

Figure 1

Carrot juice sample

14.182

17687

1.049

Figure 2

Figure 1 Chromatogram of Aspartame standard sample

Figure 2 Chromatogram of carrot juice sample


Conclusion

An effective HPLC method was developed for determination of Aspartame in carrot juice using VenusilÒ C18 Plus. Excellent separation was gained without fear of matrix influence. Typical method performance results were well within acceptable criteria.

Ordering Information

Products

Specification

Cat.No

Venusil®   C18 Plus

5 μm, 120 Å, 4.6×250 mm

VPS952505-A

1.5 mL vials

Screw neck vials,   12 × 32 mm

AV1001-6

Caps and Septa

Screw neck cap,   center hole; red silicone/ white PTEE septa, slitted

AV2200-0

Syringe Filter

Monofilm, 13 mm,   0.45 μm

AS021345-T

Disposable   Needle-Free injection systems

2 mL, 100/pk

LZSQ-2ML

 

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