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Analysis of Forsythin in Fructus Forsythia using Venusil XBP C18(L)

Application Introduction

Fructus forsythia is a Traditional Chinese Medicine with property of bitter, cool, and non-toxic. The major functions are clearing away heat and toxic materials, eliminating stagnation and reducing swelling.

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Introduction

Fructus forsythia is a Traditional Chinese Medicine with property of bitter, cool, and non-toxic. The major functions are clearing away heat and toxic materials, eliminating stagnation and reducing swelling. The product is used to alleviate the symptoms caused by Diseases epidemic febrile disease, erysipelas, macula, carbuncle, scrofula and dysuria.

Table 1 Information of Analytes

Analytes

Structure

Formula

Molecular Weigh

CAS

FORSYTHIN

C27H34O11

534.55

487-41-2

Experimental

Material and Reagents

Watsons water; HPLC grade of acetonitrile;

Fructus forsythia: provided by customer.

Sample Preparation

Transfer 1.0 g powdered fructus forsythiae and 15 mL methanol into a conical flask with cover, check the weight and impregnate overnight. Sonicate for 25 min ultrasonic treatment and keep it to the ambient. Weigh it again and add methanol for the loss weight. Evaporate 5.0 mL extract to dryness, add 0.5 g neutral alumina on neutral alumina column (from 100~120 mesh, 1g, inner diameter of 1~1.5 cm), eluted with 80mL 70% absolute ethyl alcohol. Dry the eluent under nitrogen flow and dissolve the residue with 50% methanol-water. Transfer it into 5.0 ml volumetric flask, dilute to scale, shake well. Filter the extract through 0.45 mm filter followed by HPLC analysis.

Transfer 2.0 mg reference substance of forsythin into a 10 mL volumetric flask, add methanol into it, dissolve and dilute to scale as the reference solution.

Instrumentation

HPLC Column: Venusil® XBP C18(L), 5 μm, 150 Å, 4.6 × 250 mm;

Mobile phase: water : acetonitrile = 75:25 (V/V);

Detection: 277 nm;

Flow rate: 1.0 mL/min;

Column temperature: 30 °C;

Injection: 10 mL.

Results and Discussion

Venusil® XBP C18(L) column was adopted to detect the Forsythin in Fructus forsythia. The result described that excellent separation was obtained in complex TCM matrix with sharp and symmetric peak shape.

Table 1 Performance of Venusil® XBP C18(L) in analysis of Forsythin in Fructus forsythia

Analytes

Retention   Time

Tailing   Factor

Resolution

Note

Forsythin Reference   Solution

12.070

17017

0.96

Figure 1

Extract of Fructus forsythia

12.106

19426

0.95

Figure 2

Figure 1 Chromatogram of Forsythin Reference Solution using Venusil XBP C18(L)

Figure 2 Chromatogram of the extract using Venusil XBP C18(L)

Conclusion

A robust HPLC method to analysis the Forsythin in Fructus forsythia was introduced in this paper. Venusil XBP C18(L) was adopted and excellent separation was gained with high resolution. The result shows that the Venusil XBP C18(L) was suitable for the determination of Forsythin without fear of the complex TCM matrix influence.

Ordering Information

Products

Specification

Cat.No

Venusil®   XBP C18(L)

5 μm,150 Å, 4.6 ×   250 mm

VX952505-L

Column Oven

5 - 70 °C; Max. 2 columns of 300mm

CC-100

1.5 mL vials

Screw neck vials,   12 × 32 mm

AV1001-6

Caps and Septa

Screw neck cap,   center hole; red silicone/ white PTEE septa, slitted

AV2200-0

Syringe   Filter (Nylon)

Monofilm,   13 mm, 0.22 μm

AS021320

Disposable   Needle-Free injection systems

2 mL, 100/pk

LZSQ-2ML

 

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