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Determination of Acrylamide in drinking water

Application Introduction

Acrylamide, a white crystalline chemical material, is the raw material for producing polyacrylamide. Polyacrylamide is mainly used in water purification, paper pulp processing, pipes inner coating and the polyacrylamide gel electrophoresis.

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Introduction

Acrylamide, a white crystalline chemical material, is the raw material for producing polyacrylamide. Polyacrylamide is mainly used in water purification, paper pulp processing, pipes inner coating and the polyacrylamide gel electrophoresis. Starch food at high temperature (>120℃) is prone to produce acrylamide. Research shows that acrylamide is accessible to human body through many ways, such as digestive tract, respiratory tract, skin mucous membrane and one of the most important ways is to drink water.

A survey on professionals involved in acrylamide and the group accidentally exposed to acrylamide shows that acrylamide is neurotoxic. However, there is no sufficient evidence to prove that the intake of acrylamide will lead to certain human tumor.

Experimental

Material and Regents

Watsons water; 1000 ppm Acrylamide in methanol;

Cleanert  ACA, 500mg/6mL (P/N:ACA5006);

Cleanert  ACA  1000mg/6mL (P/N:ACA0006).

* The maximum loading volumes are 500 mL and 1000 mLrespectively for the two specifications.

Sample Preparation

Activation: add 5 mL methanol and 5 mL water accordingly;

Loading: load the water sample onto Cleanert ACA cartridge;

Washing: add 5 mL water;

Dry for 5 min;

Elution: add 10 mL methanol;

Evaporate to 200 mL under 40 °C nitrogen flow, make up to 1 mL with Watsons water. Vortex well and filter the extract through 0.22 mm PTFT membrane.

Instrumentation

HPLC Column: Unisol C18,4.6mm × 250mm,5.0µm, 100Å(P/N:VA952505-0)

Mobile phase: A: Water; B: Methanol;

Detection: 210 nm;

Flow rate: 1.0 mL/min;

Column temperature: 30 °C;

Injection: 20 mL.

Table 1 Gradient

Time (min)

A%

B%

0.1

98

2

7.0

98

2

10.0

0

100

12.0

0

100

12.1

98

2

20.0

98

2

Results and Discussion

Calibration Curve

6-point calibration curve was plotted from 1 to 50 mg/mL. Take aforesaid extract for HPLC analysis, and apply internal standard methodology as quantitative method.

Quantitative of Acrylamide in drinking water:                                                

w – Quantitative of Acrylamide in drinking water, mg/g;

c – Concentration of Acrylamide in spiked sample, mg/ mL;

V – Volume of the analytical sample, mL;

m – Mass of the water sample loaded, g;

Figure 1 Calibration Curve of Acrylamide standard working solution, concentrations of Acrylamide were 1, 2, 5, 10, 20, 50μg/mL respectively.

Chromatogram


Figure 2 Chromatogram of 0.1 ppm  Acrylamide standard sample

Figure 3 Chromatogram of 0.1 ppm  Acrylamide spiked sample

Figure 4 Chromatogram of 1.0 ppm  Acrylamide standard sample

Figure 5 Chromatogram of 1.0 ppm  Acrylamide spiked sample

Figure 6 Chromatogram of Acrylamide blank sample

Table 1 Recoveries of Acrylamide spiked sample in drinking water

Sample 1

Sample 2

Sample 3

Sample 4

Sample 5

Average Recoveries

%

RSD (%, n=5)

Low concentration0.1ppm

92.5%

89.4%

90.5%

88.6%

95.8%

91.4%

3.15

High concentration1ppm

96.5%

92.6%

91.8%

89.1%

99.3%

93.9%

4.30

Conclusion

We developed an easy and efficient sample preparation procedure for the extraction of Acrylamide in drinking water. Solid phase extraction of Acrylamide from drinking water is shown with excellent recoveries and accuracy (RSD<5) using Cleanert ACA. This application demonstrated that Cleanert ACA can meet the demand of extraction of Acrylamide in drinking water.

 

Ordering Information

Products

Specification

Cat.No

Unisol C18

4.6mm ×   250mm,5.0µm, 100Å

VA952505-0

Cleanert  ACA

500mg/6mL

ACA5006

Cleanert  ACA

1000mg/6mL

ACA0006

1.5 mL vials

Screw neck vials,   12 × 32 mm

AV1001-6

Caps and Septa

Screw neck cap,   center hole; red silicone/ white PTEE septa, slitted

AV2200-0

Syringe   Filter (Nylon)

Monofilm,   13 mm, 0.45 μm

AS021345-T

Disposable   Needle-Free injection systems

2 mL, 100/pk

LZSQ-2ML



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