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Detection of 9 kinds of synthetic colorants using Venusil XBP C18(L)

Application Introduction

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Introduction

Synthetic colorants, also known as synthetic pigments, are mainly obtained from benzene, toluene and other raw materials. It is increasingly common to use colorant in modern feed industry, animal husbandry and aquaculture industry. Colorants can improve the color and value of livestock, poultry and aquatic products. For example, with colorants, the color of chicken’s skin, eggs’ yolk, butter, fish, shrimp and other aquatic products will look more fresh, bright and beautiful and customize to customers’ psychological feelings. All countries have specifically prohibited the illegal addition of non-edible colorants and excessive addition of colorants due to the fact that long-term consumption of food containing colorants can affect people’s health. The excessive pollutant in colorants can also do a damage to the main organs of human body, especially to children’s growth and development.

Table 1 Information of analytes

Analytes

Structure

Formula

Molecular Weigh

CAS

Acid   Yellow 23

 C16H9N4Na3O9S2

534.36

1934-21-0

New   red

C18H12O11N3Na3S3

611.36

220658-76-4

Acid   Red 27

C20H11N2Na3O10S3

604.47

915-67-3

Indigo

C16H10N2O2

262.26

482-89-3

Acid   Red 18

C20H11N2Na3O10S3

604.47

2611-82-7

Food   Yellow 3

C16H10N2Na2O7S2

452.37

2783-94-0

ALLURA   RED AC

C18H14N2Na2O8S2

496.42

25956-17-6

Acid   Blue 90

C47H48N3NaO7S2

854.02

6104-58-1

Acid Red 51

C20H6I4Na2O5

879.86

568-63-8

Experimental

Material and Reagents

HPLC purity of methanol; analytical pure ammonium acetate;

Instrumentation

Column: Venusil® XBP C18 (L), 5 µm, 150 Å, 4.6 × 250 mm;

Mobile phase: A: 0.02 mol/L ammonium acetate; B: methanol;

Flow rate: 1.0 mL/min;

Column temperature: 35 °C;

Injection: 10 mL;

Table 2 Gradient of elution

Time (min)

A%

B%

0.0

95

5

3.0

65

35

7.0

0

100

10.0

0

100

10.1

95

5

21

95

5

Table 3 Gradient of UV wavelength

No.

Timemin

Wavelengthnm

1

4.80

466

2

5.60

466

3

5.65

522

4

5.90

522

5

6.00

610

6

6.30

610

7

6.80

510

8

7.20

510

9

7.30

484

10

7.60

484

11

7.70

508

12

8.00

508

13

8.10

628

14

8.60

628

15

9.50

530

16

9.90

530

Results and Discussion

Vennsil XBP C18(L) column was adopted to separate and quantify 9 kinds of synthetic colorants simultaneously. The result illuminated that excellent separation was obtained with high resolution of each analyte.

Table 4 Performance of Venusil XPB C18(L) in analysis of 9 kinds of synthetic colorants

Analytes

Retention Time

Theoretical   Plates

Symmetry   Factor

Resolution

Note

Acid   Yellow 23

5.386

64265

0.95

--

Figure 1

New   red

5.520

68184

0.95

1.57

Acid   Red 27

5.728

69512

0.91

2.43

Indigo

6.074

77510

0.99

3.97

Acid   Red 18

6.949

96797

0.94

9.89

Food   Yellow 3

7.435

118063

0.96

5.51

ALLURA   RED AC

7.819

131524

0.95

4.44

Acid   Blue 90

8.458

95811

1.64

6.53

Acid Red 51

9.682

188744

0.94

12.31

Figure 1 Chromatogram of standard solution of synthetic colorants

Conclusion

A robust HPLC method to analysis of 9 kinds of synthetic colorants was introduced in this paper. And Vennsil XBP C18(L) was adopted and excellent separation was gained with high resolution. The result shows that the Vennsil XBP C18(L) was suitable for the determination of synthetic colorants in food. It’s the efficient and powerful tool for QC test of food manufacturers and routine analysis of government regulatory.

Ordering Information

Products

Specification

Cat.No

Venusil®   XBP C18(L)

5 μm, 150 Å,   4.6×250 mm

VX952505-L

1.5 mL vials

Screw neck vials,   12 × 32 mm

AV1001-6

Caps and Septa

Screw neck cap,   center hole; red silicone/ white PTEE septa, slitted

AV2200-0

Syringe   Filter (Nylon)

Monofilm,   13 mm, 0.22 μm

AS021320

Disposable   Needle-Free injection systems

2 mL, 100/pk

LZSQ-2ML


 

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