Aela Agela Technologies

Advance search
Quick Order
See your previous items:
You have 0 product(s) in cart

Analysis of Phenoxyethanol and Nipagin in cosmetics using Bonshell Polar Phenyl

Application Introduction

Favorite

Introduction

Phenoxyethanol is a kind of colorless, slightly sticky, liquid and organic compound. It smells fragrant and tastes astringent. It can be fully mixed with acetone, ethanol and glycerin and slightly dissolve in water. Phenoxyethanol is a common and relatively safe preservative in cosmetics and the maximum concentration in cosmetics is limited to 1%.

Nipagin esters, also called paraben, is a better preservative than benzoic and its salts due to the higher antibacterial property and lower toxicity. Nipagin esters’ bacteriostasis is little impacted by pH, so it is often added in food, medicine and cosmetics as preservative.

In this paper, an efficient analytical method was introduced in determination of those two kinds of preservatives using Bonshell Polar Phenyl.

Table 1 Information of analytes

Analytes

Structure

Formula

Molecular Weigh

CAS

Phenoxyethanol

 C8H10O2

138.16

122-99-6

Methylparaben

C8H8O3

152.25

99-76-3

Ethylparaben

C9H10O3

166.17

118-61-6

Isopropylparaben

C10H12O3

180.2

4191-73-5

Propylparaben

C10H12O3

180.2

94-13-3

Isobutylparaben

C11H14O3

194.23

4247-02-3

Butylparaben

C11H14O3

194.23

94-26-8

Experimental

Material and Reagents

Watsons water; HPLC purity of methanol and potassium dihydrogen phosphate;

Standard solution of 7 preservatives mix: provided by customer;

Instrumentation

Column: Bonshell Polar Phenyl; 2.7 μm, 100 Å, 4.6 × 100 mm;

Mobile phase: 10 mmol/L of potassium dihydrogen phosphate : methanol = 60:40 (V/V);

Detection: 257 nm;

Flow rate: 1.0 mL/min;

Column temperature: 40 °C;

Injection: 5 mL.

Results and Discussion

We developed a robust analytical method for quantifying 7 kinds of preservatives in cosmetics. Excellent chromatographic separation was obtained using Bonshell Polar Phenyl for each analyte.

Table 2 Performance of Bonshell Polar Phenyl in analysis of standard solution of 7 preservatives mixed

Analytes

Retention   Time

Number   of Theoretical Plates

Tailing   Factor

Resolution

Notes

Component1

2.091

10902

1.039

-

Figure 1

Component2

2.374

9339

1.042

3.14

Component3

3.738

10736

1.022

11.13

Component4

5.742

11767

1.015

11.16

Component5

6.648

13076

1.043

4.02

Component6

11.515

13452

1.016

15.15

Component7

12.823

13515

1.001

3.10

Figure 1 Chromatography of 7 preservatives standard solution mixed

Conclusion

We developed a robust analytical method for quantifying 7 kinds of preservatives in cosmetics. Excellent chromatographic separation was obtained using Bonshell Polar Phenyl for each analyte. Bonshell Polar Phenyl delivered robustness performance and thoroughly meet the demand of safety and regulatory testing.

Ordering Information

 

Products

Specification

Cat.No

Bonshell®   Polar Phenyl

2.7 μm,100 Å,   4.6×100 mm

SXP621005-0

1.5 mL vials

Screw neck vials,   12 × 32 mm

AV1001-6

Caps and Septa

Screw neck cap,   center hole; red silicone/ white PTEE septa, slitted

AV2200-0

Syringe   Filter (Nylon)

Monofilm,   13 mm, 0.45 μm

AS021345-T

Disposable   Needle-Free injection systems

2 mL, 100/pk

LZSQ-2ML

Download
Material Name Material Type Date
Modify product number success!
Total Price:

Close

Email To Sombody

X
Email
Subject
Content

Login

X
Email address
Password
Register Forgotten your password?