Asiaticoside(AS) is the triterpenoid saponins compound effectively extracted from the centella asiatica which is an umbelliferous plant. Asiaticoside mainly contains asiaticoside, madecassoside and their homologs, and has pharmacological action in immunoregulation, anti-inflammation, etc.
Table 1 Information of Analytes
Material and Reagents
Watsons water; HPLC grade of acetonitrile;
Standard solution of Asiaticoside B and Madecassoside: provided by customer.
HPLC Column: Bonshell® Polar Phenyl, 2.7 μm, 100 Å, 4.6 × 100 mm;
Mobile phase: acetonitrile : water = 20:80 (V/V);
Detection: 205 nm;
Flow rate: 1.0 mL/min;
Column temperature: 30 °C;
Injection: 10 mL.
Results and Discussion
Bonshell® Polar Phenyl column was adopted to separate and quantify the Asiaticoside B and Madecassosid components. The result illuminated that excellent separation was obtained with the resolution of 1.91. Robustness delivered the analysis of homologs of Asiaticoside in natural product drug discovery and regulatory.
Table 1 Performance of Bonshell Polar Phenyl in analysis of standard solution of Asiaticoside B and Madecassosid
Figure 1 Chromatogram of standard solution of Asiaticoside B and Madecassosid using Bonshell® Polar Phenyl
A robust HPLC method to analysis Asiaticoside B and Madecassosid components was introduced in this paper. And Bonshell® Polar Phenyl was adopted and excellent separation was gained with high resolution. The result shows that the Bonshell® Polar Phenyl was suitable for the determination of homologs of Asiaticoside, efficient and powerful.
Bonshell® Polar Phenyl
2.7 μm,100 Å, 4.6 × 100 mm
1.5 mL vials
Screw neck vials, 12 × 32 mm
Caps and Septa
Screw neck cap, center hole; red silicone/ white PTEE septa, slitted
Syringe Filter (Nylon)
Monofilm, 13 mm, 0.22 μm
Disposable Needle-Free injection systems
2 mL, 100/pk