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Analysis of Asiaticoside B and Madecassoside

Application Introduction

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Introduction

Asiaticoside(AS) is the triterpenoid saponins compound effectively extracted from the centella asiatica which is an umbelliferous plant. Asiaticoside mainly contains asiaticoside, madecassoside and their homologs, and has pharmacological action in immunoregulation, anti-inflammation, etc.

Table 1 Information of Analytes

Analytes

Structure

Formula

Molecular Weigh

CAS

Asiaticoside   B

C48H78O20  

975.12

125265-68-1

Madecassoside

C48H78O20

975.12

34540-22-2

Experimental

Material and Reagents

Watsons water; HPLC grade of acetonitrile;

Standard solution of Asiaticoside B and Madecassoside: provided by customer.

Instrumentation

HPLC Column: Bonshell® Polar Phenyl, 2.7 μm, 100 Å, 4.6 × 100 mm;

Mobile phase: acetonitrile : water = 20:80 (V/V);

Detection: 205 nm;

Flow rate: 1.0 mL/min;

Column temperature: 30 °C;

Injection: 10 mL.

Results and Discussion

Bonshell® Polar Phenyl column was adopted to separate and quantify the Asiaticoside B and Madecassosid components. The result illuminated that excellent separation was obtained with the resolution of 1.91. Robustness delivered the analysis of homologs of Asiaticoside in natural product drug discovery and regulatory.

Table 1 Performance of Bonshell Polar Phenyl in analysis of standard solution of Asiaticoside B and Madecassosid

Analytes

Retention   Time

Tailing   Factor

Resolution

Note

Asiaticoside B

6.726

1.055

--

Figure 1

Madecassosi

7.174

0.980

1.91

Figure 1 Chromatogram of standard solution of Asiaticoside B and Madecassosid using Bonshell® Polar Phenyl

Conclusion

A robust HPLC method to analysis Asiaticoside B and Madecassosid components was introduced in this paper. And Bonshell® Polar Phenyl was adopted and excellent separation was gained with high resolution. The result shows that the Bonshell® Polar Phenyl was suitable for the determination of homologs of Asiaticoside, efficient and powerful.

Ordering Information

Products

Specification

Cat.No

Bonshell®   Polar Phenyl

2.7 μm,100 Å, 4.6   × 100 mm

SXP621005-0

1.5 mL vials

Screw neck vials,   12 × 32 mm

AV1001-6

Caps and Septa

Screw neck cap,   center hole; red silicone/ white PTEE septa, slitted

AV2200-0

Syringe   Filter (Nylon)

Monofilm,   13 mm, 0.22 μm

AS021320

Disposable   Needle-Free injection systems

2 mL, 100/pk

LZSQ-2ML

 

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