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Analysis of 7 kinds of antibiotics in cosmetics using Unisol C18(2)

Application Introduction

The application note demonstrated the analysis of multi antibiotics in cosmetics using Unisol C18(2) column.

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The application note demonstrated the analysis of multi antibiotics in cosmetics using Unisol C18(2) column.

Table 1 Information of analytes

Analytes

Formula

Molecular Formula

Molecular Weight

CAS

Minocycline   hydrochloride

  C23H28 ClN3O7

493.9373

13614-98-7

Metronidazole

C6H9N3O3

171.154

443-48-1

Oxytetracycline

 C22H26N2O8

446.4504

79-57-2

Tetracycline   Hydrochloride

C22H25   ClN2O8

480.8955

64-75-5

Aureomycin   hydrochloride

C22H24Cl2N2O8

515.3406

64-72-2

Doxycycline   hydrochloride

C22H25ClN2O8

480.8955

10592-13-9

Chloroamphenicol

C11H12Cl2N2O5

323.1294

56-75-7

Experimental

Material and Reagents

Watsons water; HPLC purity of methanol, acetonitrile and TFA;

Reference solution of 7 kinds of antibiotics mix: provided by customer;

Facial cleanser: bought from supermarket;

Blank sample: Transfer 1 g facial cleanser into a 10 mL conical flask with cover, add 10 mL methanol – 0.1 mol/L HCl (1:1, V/V) and sonicate for 30 min, filter the extract through 0.45 mm filter;

Blend of blank sample and reference solution: Take 100 mL blank sample solution and 100 mL reference solution mixed respectively, mix well.

Instrumentation

Column: Unisol C18(2), 5 µm, 110 Å, 4.6 × 250 mm;

Mobile phase: A: acetonitrile; B: 0.1 mol/L TFA in water; C: methanol;

Detection: 268 nm;

Flow rate: 0.8 mL/min;

Column temperature: 35 °C;

Injection: 5 mL;

Table 2 Gradient

Time(min)

A%

B%

C%

0

10

85

5

5

20

70

10

15

20

40

40

25

30

40

30

30

10

85

5

35

10

85

5

Results and Discussion

We developed a robust analytical method for quantifying 7 kinds of antibiotics in cosmetics. Excellent chromatographic separation was obtained using Unisol C18(2) for each analyte.

Table 3 Performance of Unisol C18(2) in analysis of reference solution of 7 antibiotics mixed

Analytes

Retention   Time

Number   of Theoretical Plates

Tailing   Factor

Resolution

Notes

Minocycline   hydrochloride

6.359

28268

0.95

--

Figure 1

Metronidazole

8.942

65047

1.28

17.69

Oxytetracycline

11.275

94561

0.87

16.24

Tetracycline   Hydrochloride

12.074

91247

0.90

5.21

Aureomycin   hydrochloride

15.436

94968

0.78

18.63

Doxycycline   hydrochloride

16.281

129018

0.96

4.42

Chloroamphenicol

17.267

133754

0.69

5.32

Table 4 Performance of Unisol C18(2) in analysis of spiked sample

Analytes

Retention   Time

Number   of Theoretical Plates

Tailing   Factor

Resolution

Notes

Minocycline   hydrochloride

6.356

28649

0.95

--

Figure 3

Metronidazole

8.922

66847

1.30

17.78

Oxytetracycline

11.256

93761

0.87

16.35

Tetracycline   Hydrochloride

12.058

88558

0.87

5.18

Aureomycin   hydrochloride

15.429

95068

0.75

18.59

Doxycycline   hydrochloride

16.266

132152

0.92

4.41

Chloroamphenicol

17.259

128520

0.66

5.33

Figure 1 Chromatogram of reference solution mix of 7 kinds of antibiotics

Figure 2 Chromatogram of blank sample

Figure 3 Chromatogram of spiked sample in facial cleanser

 

Conclusion

We developed a robust analytical method for quantifying 7 kinds of antibiotics in cosmetics. Excellent chromatographic separation was obtained using Unisol C18(2) for each analyte. The result described that the analytes were not detected in the facial cleanser samples and there was no interference of matrix for the separation of antibiotics. Unisol C18(2) delivered robustness performance and thoroughly meet the demand of safety and regulatory testing.

Ordering Information

Products

Specification

Cat.No

Unisol   C18(2)

5 μm,110 Å, 4.6×250 mm

VA952505-2

1.5 mL vials

Screw neck vials,   12 × 32 mm

AV1001-6

Caps and Septa

Screw neck cap,   center hole; red silicone/ white PTEE septa, slitted

AV2200-0

Syringe   Filter (Nylon)

Monofilm,   13 mm, 0.45 μm

AS021345-T

Disposable   Needle-Free injection systems

2 mL, 100/pk

LZSQ-2ML


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