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Detection of β-carotene in milk powder

Application Introduction

β-carotene, an orange fat-soluble compound, is a kind of carotenoid and it is the most common and most stable natural pigment in nature. β-carotene is inevitable for maintaining health for antioxidation and detoxification.

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Introduction

β-carotene, an orange fat-soluble compound, is a kind of carotenoid and it is the most common and most stable natural pigment in nature. β-carotene is inevitable for maintaining health for antioxidation and detoxification. It plays a remarkable role in anti-cancer, cardiovascular diseases and cataract prevention, anti-oxidation and the prevention of degenerative diseases caused by aging.

Table 1 Information of Analyte

Name

Structure

Formula

Molecular Weight

CAS

β-carotene

 C40H56

536.8726

7235-40-7

Experimental

Material

HPLC purity of methanol and acetonitrile; analytical pure chloroform; analytical pure ascorbic acid; β-carotene standard sample solution and sample solution (provided by customers).

Instrumentation

HPLC Column: Innoval ODS-2; 5 μm, 100 Å, 4.6 × 250 mm;

Mobile phase: Chloroform : Acetonitrile : Methanol = 3:12:85, containing 0.4 g/L ascorbic acid;

Detection: 450 nm;

Flow rate: 2.0 mL/min;

Column temperature: 35 °C;

Injection: 20 mL.

Results and Discussion

Innoval ODS-2 HPLC column was adopted to detect the β-carotene standard samples and samples in the experiment. Table 2 and the following Figure show that the result can meet detesting demand.

Table 2 Performance of Innoval ODS-2 in detection β-carotene in milk powder sample

 

Sample

Analytes

Retention Time

Number of theoretical number

Tailing factor

Notes

Standard sample of β-carotene

β-carotene

8.995

7996

0.91

Figure 1

Milk powder sample

β-carotene

8.981

7069

0.86

Figure 2

Figure 1 Chromatogram of β-carotene in standard sample

Figure 2 Chromatogram of β-carotene in milk powder sample

Conclusion

The β-carotene standard sample and milk powder sample were detected by Innoval ODS-2 HPLC column in the experiment. The result shows that the Innoval ODS-2 HPLC column can meet the testing requirement.

Ordering Information

Products

Specification

Cat.No

Innoval ODS-2

5 μm, 100   Å, 4.6×250 mm

IX952505-2

1.5 mL vials

Screw neck vials,   12 × 32 mm

AV1001-6

Caps and Septa

Screw neck cap,   center hole; red silicone/ white PTEE septa, slitted

AV2200-0

Syringe   Filter (Nylon)

Monofilm,   13 mm, 0.22 μm

AS021320

Disposable   Needle-Free injection systems

2 mL, 100/pk

LZSQ-2ML

 

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