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Detection of Chlorophenol in the Water

Application Introduction

Chlorophenols (CPs) exist in waste water of chemical and paper-making industries.

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Introduction

Chlorophenols (CPs) exist in waste water of chemical and paper-making industries. Most of CPs with strong toxicity are difficult to be biodegraded, and can accumulate in organisms. Therefore, CPs have been listed as one of main organic pollutants by EPA of America. This paper conducts the sample pretreatment to five kinds of CPs in river water by Qdaura® Automatic SPE System, and compares it with the manual pretreatment method.

Experimental

Materials

SPE Cartridge: Cleanert® PEP (500 mg / 6 mL)

Adjust the pH of 500 mL of water sample with 0.5 mol/L H2SO4 to 1.5~2.0.

SPE Method

Activation: activate the PEP cartridge with 5 mL of methanol and 5 mL of water sequentially.

Sample loading: load water sample onto the cartridge at a flow rate of below 5 mL/min.

Washing: Wash the cartridge with 5 mL of pure water and dry the cartridge under nitrogen stream for 20 min. Wash the cartridge with 0.8 mL of methanol (stand for 2 min to ensure methanol to soak packing material thoroughly) and discard the eluate.

Elution: Elute the cartridge with 5 mL of furanidine. Collect the eluate and concentrate under nitrogen stream at 30 °C to 1 mL for HPLC determination.

Instrumentation

Column: Unisol C18, 4.6 mm × 150 mm, 5 μm, 100 Å

Mobile phase: A, water with 0.1% formic acid; B, methanol with 0.1% formic acid

Injection: 20 μL

Temperature: 30 °C

Detection UV: 285 nm

Table 1 Gradient Elution Condition

Time/min

Flow rate (mL/min)

A/%

B/%

0

1

35

65

6

1

35

65

15

1.8

5

95

15.1

1

35

65

22

1

35

65

Results and Discussion

Table 2 Recoveries and reproducibility of spiked water sample (n=3, 1 μg/L)

Analytes

Manual Method

Qdaura Method

Recoveries/%

RSD/%

Recoveries/%

RSD/%

1-Chlorophenol

78.89

6.76

76.38

4.46

2-Chlorophenol

82.44

7.47

89.51

1.99

3-Chlorophenol

60.96

10.24

71.15

3.29

4-Chlorophenol

86.04

9.07

76.63

2.3

5-Chlorophenol

86.63

10.01

89.01

4.81

 

Figure 1 Chromatogram of spiked water sample (1 μg/L)

Conclusions

It is shown by the test results that the recovery and RSD of spiked samples from river water through the sample pretreatment by a full-automatic solid-phase extractor are significantly superior to those through the manual solid-phase extraction.

Order Information

Products

Specification

Cat.No

Cleanert®   PEP

500 mg / 6 mL

PE5006

Qdaura®   Automatic SPE System

4 channels, 24   positions

SPE-40

 


  • Qdaura Automated SPE System

    The Qdaura Automated SPE system is specifically designed for high throughput sample preparation. The system automates routine SPE procedures from conditioning, sample loading, washing, dry and elution. The Qdaura utilizes positive pressure to ensure more reproducible flow rates, more reproducible recoveries, less sample to sample contamination, and higher precision.

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